Kales (Brassica oleracea) are a staple leafy vegetable in Kenya, consumed widely for their nutritional benefits. Nakuru County is a significant horticultural hub, with numerous small to large-scale farms cultivating kales. However, the increasing use of agrochemicals, coupled with industrial activities, raises concerns about the potential contamination of soil and crops by heavy metals. Therefore, this study determined the levels of selected heavy metals in samples of kales obtained from horticultural farms in Nakuru County, Kenya. A total of 120 kales (Sukuma wiki) leaves were randomly collected from Njoro, Molo, Naivasha, Nakuru East, Nakuru West, Subukia, Rongai and Gilgil sub-counties within the study area and transported to University of Eldoret Chemistry lab for sample preparation. There, the leaves samples were air dried, grinded and digested. Analysis was conducted using Shimadzu AAS model SpectrAA-200. Data generated was analysed descriptively and inferentially using SPSS. The study found that the concentrations of selected heavy metals (Cr, Fe, Cu, Mn, and Zn) in kales from various horticultural farms in Nakuru County were below the WHO/FAO permissible limits. These findings suggest that kales grown in this region are safe for consumption regarding these specific heavy metals. Further, there was a significant difference in Cu, Zn, Cr and Fe across 8 regions (P<0.05). The overall mean levels of heavy metals in the kales followed the order: Cr ˃ Fe ˃ Zn ˃ Mn ˃ Cu, with chromium being the most prevalent. Despite the current safety, continuous monitoring is important to ensure that heavy metal levels remain within safe limits, considering the ongoing use of agrochemicals and potential industrial pollution. Also, it is recommended that local authorities and agricultural stakeholders implement regular monitoring programs to track heavy metal concentrations in kales and other vegetables.
References
Akubugwo, E. I., Obasi, A., Chinyere, G. C., Eze, E., Nwokeoji, O. and Ugbogu, E. A. (2012). Phytoaccumulation effects of Amaranthus hybridus L. grown on buwaya refuse dumpsites in Chikun, Nigeria on 105 heavy metals. Journal of Biodiversity and Environmental Sciences (JBES), 2(5), 10–17.
Alamin, M.B., Mhapes, A.A., Bejey, A.M., Sadek, A., Atweer, R.H., Dubali, K., and Saad, D.M., (2007), Determination of essential and toxic elements in Libyan foodstuff using instrumental neutron activation analysis (INAA). Journal of Radioanalytical and Nuclear Chemistry 271, p. 247–250
Ali, H., Khan, E., & Ilahi, I. (2019). Environmental chemistry and ecotoxicology of hazardous heavy metals: environmental persistence, toxicity, and bioaccumulation. Journal of chemistry, 2019.
Alves, L. R., Reis, A. D., & Gratão, P. L. (2016). Heavy metals in agricultural soils: from plants to our daily life (a review).
Aweng, E. R., Karimah, M., and Suhaimi, O. (2011). Heavy metals concentration of irrigation water, soils and fruit vegetables in Kota Bharu area, Kelantan, Malaysia. J. Appl. Sci. Environ. Sanit, 6(4), 463–470.
Bett, L., Ongera, G., Wangila, P., & Mule, S. (2019). Determination of some heavy metals in soils and vegetables amples from Kericho West Sub-County, Kenya.
Bhargava, P., Gupta, N., Vats, S., & Goel, R. (2017). Health issues and heavy metals. Austin J Environ Toxicol, 3(1), 3018.
County, N. (2015). Nakuru County Integrated Development Plan 2013–2017.
Foeken, D. W. J., Owuor, S. O., & Klaver, W. (2002). Crop cultivation in Nakuru town, Kenya: Practice and potential.
Gonçalves Jr, A. C., Nacke, H., Schwantes, D., & Coelho, G. F. (2014). Heavy metal contamination in brazilian agricultural soils due to application of fertilizers. Environmental risk assessment of soil contamination, 4(1), 105-135.
Gupta, N., Yadav, K. K., & Kumar, V. (2015). A review on current status of municipal solid waste management in India. Journal of environmental sciences, 37, 206-217.
Hellen, L. E., and Othman, O. C. (2014). Levels of selected heavy metals in soil, tomatoes and selected vegetables from Lushoto district-Tanzania. International Journal of Environmental Monitoring and Analysis, 2(6), 313–319.
Inoti, K. J., Fanuel, K., George, O., & Paul, O. (2012). Assessment of heavy metal concentrations in urban grown vegetables in Thika Town, Kenya. African Journal of Food Science, 6(3), 41-46.
Jaishankar, M., Tseten, T., Anbalagan, N., Mathew, B. B., & Beeregowda, K. N. (2014). Toxicity, mechanism and health effects of some heavy metals. Interdisciplinary toxicology, 7(2),60-72.
Karanja, N. N., Njenga, M., Mutua, G. K., Lagerkvist, C. J., & Okello, J. J. (2012). Concentrations of heavy metals and pesticide residues in leafy vegetables and implications for peri-urban farming in Nairobi, Kenya. Journal of Agriculture, Food Systems, and Community Development, 3(1), 255-267
Khan, S., Cao, Q., Zheng, Y. M., Huang, Y. Z., & Zhu, Y. G. (2008). Health risks of heavy metals in contaminated soils and food crops irrigated with wastewater in Beijing, China.Environmental Pollution,
Kinambuga, D. (2010). Evaluation of the constraints to profitable smallholder dairying; a case of nakuru county kenya (Doctoral dissertation, Egerton University).
Lyimo, M., Temu, R. P. C., & Mugula, J. K. (2003). Identification and nutrient composition of indigenous vegetables of Tanzania. Plant Foods for Human Nutrition, 58, 85-92.
Manzoor, J., Sharma, M., & Wani, K. A. (2018). Heavy metals in vegetables and their impact on the nutrient quality of vegetables: A review. Journal of plant Nutrition, 41(13), 1744-1763.
Mishra, S., Bharagava, R. N., More, N., Yadav, A., Zainith, S., Mani, S., & Chowdhary, P. (2019). Heavy metal contamination: an alarming threat to environment and human health. Environmental biotechnology: For sustainable future, 103-125.
Muhammad, F., Anwar, F. and Rashid, U. (2008). Appraisal of Heavy Metal Contents in Different Vegetables Grown in the Vicinity of an Industrial Area. Pakistan Journal of Botany, 40(5), 2099–2106
Muojiama, S. O., Nwune, U. C., Ugo, G. O., Ezeh, M. C., & Ukwu, U. N. (2023). Growth performance and nutritional concentrations of two kale (Brassica oleracea var Acephala) varieties in response to fertilizer types in Awka, Southeast Nigeria. International Journal of Recycling Organic Waste in Agriculture, 12(4), 723-733.
Nachana’a Timothy, E. T. W. (2019). Environmental pollution by heavy metal: an overview. Chemistry, 3(2), 72-82.
Njagi, J.M. (2013). Assessment of Heavy Metal Concentration in the Environment and Perceived Health Risks by the Community around Kadhodeki dumpsite, Nairobi County. (Msc) Kenyatta University.
Ratul, A. K., Hassan, M., Uddin, M. K., Sultana, M. S., Akbor, M. A., & Ahsan, M. A. (2018). Potential health risk of heavy metals accumulation in vegetables irrigated with polluted river water. International food research journal, 25(1).
Sandeep, G., Vijayalatha, K. R., & Anitha, T. (2019). Heavy metals and its impact in vegetable crops. Int J Chem Stud, 7(1), 1612-21.
Satheesh, N., & Workneh Fanta, S. (2020). Kale: Review on nutritional composition, bio-active compounds, anti-nutritional factors, health beneficial properties and value-added products. Cogent Food & Agriculture, 6(1), 1811048.
Uwah, E. I., Ndahi, N. P., Abdulrahman, F. I. and Ogugbuaja, V.O. (2011). Heavy metal levels in spinach (Amaranthus caudatus) and lettuce (Lactuca sativa) grown in Maiduguri, Nigeria. Journal of Environmental Chemistry and Ecotoxicology, 3(10), 264–271.
WHO/FAO, Codex Alimentarius Commission (2001). Food additives and contamination. Joint FAO/WHO Food Standards programme; p 45 – 48.
Witkowska, D., Słowik, J., & Chilicka, K. (2021). Heavy metals and human health: Possible exposure pathways and the competition for protein binding sites. Molecules, 26(19), 6060.
Zahra, N., & Kalim, I. (2017). Perilous effects of heavy metals contamination on human health. Pakistan Journal of Analytical & Environmental Chemistry, 18(1), 1-17.